Sequence-tagged connectors: A sequence approach to mapping and scanning the human genome

Sequence-tagged connectors: a sequence approach to mapping and scanning the human genome.

The sequence-tagged connector (STC) strategy proposes to generate sequence tags densely scattered (every 3.3 kilobases) across the human genome by arraying 450,000 bacterial artificial chromosomes (BACs) with randomly cleaved inserts, sequencing both ends of each, and preparing a restriction enzyme fingerprint of each. The STC resource, containing end sequences, fingerprints, and arrayed BACs, ...

Rice transposable elements: a survey of 73,000 sequence-tagged-connectors.

As part of an international effort to sequence the rice genome, the Clemson University Genomics Institute is developing a sequence-tagged-connector (STC) framework. This framework includes the generation of deep-coverage BAC libraries from O. sativa ssp. japonica c.v. Nipponbare and the sequencing of both ends of the genomic DNA insert of the BAC clones. Here, we report a survey of the transpos...

Optimized strategies for sequence-tagged-site selection in genome mapping.

The physical mapping of complex genomes is based on the construction of a genomic library and the determination of the overlaps between the inserts of the mapping clones in order to generate an ordered, cloned representation of nearly all the sequences present in the target genome. Evaluation of the relative efficiency of experimental procedures used to accomplish this goal must minimally inclu...

A User's Guide to the Human Genome. Question 2. How can sequence-tagged sites within a DNA sequence be identified?

Mapping Sequence diagram in Fuzzy UML to Fuzzy Petri Net

This ability in fuzzy UML, practically leaves the customers and market’s need without response in this important and vital area. Here, the available sequence diagrams in fuzzy UML will map into fuzzy Petri net. However, the formal models ability will be added to the Semi-formal fuzzy UML. This formalization will add the automatic processing ability to the Semi-formal fuzzy UML. Further more, t...